10.22376/ijpbs.2019.10.1.p1-12 Volume 7 Issue 4 2016 (October - December) Root nodulating bacteria was isolated from root nodules of lessThan i greaterThan Vigna trilobata lessThan /i greaterThan (L) Verdc. Plants raised in the soils were collected from five geographically different areas in Andhra Pradesh, India. The bacteria was identified as lessThan i greaterThan Agrobacterium lessThan /i greaterThan lessThan i greaterThan tumefaciens lessThan /i greaterThan strains by 16S rDNA analysis. Except lessThan i greaterThan A. tumefaciens lessThan /i greaterThan MRR 102, all the strains produced round shape and white coloured colonies with lot of mucilage on YEMA medium. All the lessThan i greaterThan Agrobacterium lessThan /i greaterThan strains grew well on YEM medium with NaCl (0.1%), pH (7.0), and at temperature 35⁰C. All the five strains were positive for biochemical tests such as Ammonia production test, catalase test, oxidase test, nitrate reduction test, and urease test. All these strains showed negative results on Hoffer's Alkaline medium, Ketolactose test, and Glucose peptone Agar medium. lessThan i greaterThan A. tumefaciens lessThan /i greaterThan MRR 102 showed the negative results on amylase test and the remaining isolates showed positive. For citrate utilization except lessThan i greaterThan A. tumefaciens lessThan /i greaterThan MRR 115, all the strains showed the positive results. Plant growth promoting characteristics such as Phosphate solubilization, IAA, EPS chitinase, siderophore and HCN production were estimated for these lessThan i greaterThan Agrobacterium lessThan /i greaterThan strains. IAA and EPS production was observed in all these strains with a range of 30-120 µg/ml and 402-569 mg/ml respectively. Two out of five strains i.e. lessThan i greaterThan A. lessThan /i greaterThan lessThan i greaterThan tumefaciens lessThan /i greaterThan MRR 102 and lessThan i greaterThan A. lessThan /i greaterThan lessThan i greaterThan tumefaciens lessThan /i greaterThan  MRR 105 showed the phosphate solubilization. Only one strain lessThan i greaterThan A. lessThan /i greaterThan lessThan i greaterThan tumefaciens lessThan /i greaterThan  MRR 111 showed the chitinase production (0.20 U/ml) on chitin agar medium. Two out of five lessThan i greaterThan Agrobacterium lessThan /i greaterThan sp. namely MRR 102 and 105 showed the Siderophore production. The two strains lessThan i greaterThan A. tumefaciens lessThan /i greaterThan MRR 108 and lessThan i greaterThan A. tumefaciens lessThan /i greaterThan MRR 111 showed the positive results for HCN production. Among the five strains, lessThan i greaterThan A. lessThan /i greaterThan lessThan i greaterThan tumefaciens lessThan /i greaterThan MRR 111 and lessThan i greaterThan A. lessThan /i greaterThan lessThan i greaterThan tumefaciens lessThan /i greaterThan MRR 102 strains was found to most effective in positively enhancing the growth and the plant growth promoting characteristics in all parameters. G. KRANTHI KUMAR AND M. RAGHU RAM Agrobacterium tumefaciens, Yeast Extract Mannitol Agar, Vigna trilobata. 532-539