10.22376/ijpbs.2019.10.1.p1-12 Volume 7 Issue 4 2016 (October - December) To evaluate the synergistic potency of three different plants namely rhizome of lessThan i greaterThan Bergenia ciliata, Acorus calamus lessThan /i greaterThan and tuber of lessThan i greaterThan Dioscorea bulbifera lessThan /i greaterThan in exhibiting the antibacterial activity, phytochemical evaluation and molecular docking analysis. 5mg/ml, Sample II and III acetone extract of plant mixture containing lessThan i greaterThan A. calamus, B. ciliata lessThan /i greaterThan  and lessThan i greaterThan D. bulbifera lessThan /i greaterThan in the ratio 1:2:1 and 1:1:2 against lessThan i greaterThan E. coli, lessThan /i greaterThan showed 12mm and 13mm lessThan i greaterThan , P. aeruginosa lessThan /i greaterThan showed 13mm and 16mm and lessThan i greaterThan B. subtilis lessThan /i greaterThan showed 23mm and 18mm zone of inhibition. To identify, the chemical compounds responsible for antimicrobial activity the acetone extract of sample II and III which was analysed using GC- lessThan i greaterThan MS and Molecular Docking lessThan /i greaterThan . Compared to other metabolites alpha.- (N,N-Dimethylamino)-3'-hydroxy-4'-methoxyacetophenone and Cis-Asarone are most potent metabolites which has lowest docking score against the receptors at various degrees. The results will be useful for further design of novel cancer and microbial inhibitors derived from synergistic plant extract. K. GOWRI SHANKAR, ALBIN T FLEMING, R.VIDHYA AND NAMRATA PRADHAN Preliminary Phytochemical Screening, Antimicrobial Activity,GC-MS Analysis, Synergy, Molecular Docking. 619-628