10.22376/ijpbs.2019.10.1.p1-12 Volume 7 Issue 4 2016 (October - December) Our aims is to clone the Glucose oxidase (GOx) gene which has been isolated from lessThan i greaterThan Aspergillus niger lessThan /i greaterThan , in turn isolated from the fertile garden soil. Detection of the expression of lessThan i greaterThan gox lessThan /i greaterThan gene in prokaryotic host. lessThan i greaterThan Aspergillus niger lessThan /i greaterThan has been isolated from soil samples taken from Navsari region. Enzyme assay has been done to check enzyme. DNA extraction & PCR amplification was done using Banglore GeNei lessThan sup greaterThan TM lessThan /sup greaterThan kits. 90% success has been achieved by obtaining the GOx protein from lessThan i greaterThan Escherichia coli lessThan /i greaterThan BL21 in inclusion bodies. The protein has been purified onto Ni-NTA column. lessThan i greaterThan gox lessThan /i greaterThan gene was successfully isolated and expressed in prokaryotic host, which would be purified by simple procedure. –We conclude that the by-product of gluconic acid fermentation has a great industrial importance. So, for greater production of GOx, the gene can be transferred from donor to the recipient. The lessThan i greaterThan gox lessThan /i greaterThan gene of lessThan i greaterThan Aspergillus niger lessThan /i greaterThan has been isolated and studied for the identification & its expression for improvement of enzyme production by establishing a process based on heterologous expression. SUMAIYA SHAIKH AND RATNA TRIVEDI Glucose oxidase (Gox), Aspergillus niger, cloning, expression. 685-688