10.22376/ijpbs.2019.10.1.p1-12 Volume 7 Issue 4 2016 (October - December) In present study highly sensitive and simple spectrophotometric method reported in the literature was modified and employed for speciation of selenium in medicinal plants. Total Selenium and bioaccessible selenium after lessThan i greaterThan in-vitro lessThan /i greaterThan gastric and gastrointestinal method was determined using spectrophotometer and results were also verified with Inductively coupled plasma atomic emission spectroscopy (ICP-AES) technique. The method involves reaction of selenium (IV) with 2, 3 di-aminonapthalene to give the bright colored and strongly fluorescent 4, 5-benzopiazselenol compound. The optimum pH for this reaction was 2 and optimum temperature was 50 lessThan sup greaterThan 0 lessThan /sup greaterThan C. The molar absorptivity of the complex formed was 29.866 × 10 lessThan sup greaterThan 3 lessThan /sup greaterThan L mol lessThan i greaterThan lessThan sup greaterThan − lessThan /sup greaterThan lessThan /i greaterThan lessThan sup greaterThan 1 lessThan /sup greaterThan cm lessThan sup greaterThan -1 lessThan /sup greaterThan . This method is applicable in the range of 25 - 2000 µg/l of selenium. Highest selenium content was found to be 32 µg/g in lessThan i greaterThan Amoora rohitaka (Roxb). lessThan /i greaterThan An lessThan i greaterThan in-vitro lessThan /i greaterThan study indicates that bioaccessibility of selenium in lessThan i greaterThan Sphaeranthus hirtus willed lessThan /i greaterThan was highest during gastro-intestinal digestion. This result shows that medicinal plants can be a good source of selenium bioaccessibility being high. KUNDA N. GAIKWAD AND NILIMA S. RAJURKAR Selenium, speciation, bioaccessibility, ICP-AES, medicinal plants 132-138