10.22376/ijpbs.2019.10.1.p1-12 Volume 7 Issue 4 2016 (October - December) In the present work, a unique attempt has been made to extract from lessThan i greaterThan in vivo lessThan /i greaterThan and lessThan i greaterThan in vitro lessThan /i greaterThan plant parts along with callus and undergone HPTLC (High Performance Thin Layer Chromatography) analysis to quantify stigmasterol from lessThan i greaterThan Chlorophytum borivilianum lessThan /i greaterThan Santapau & R. R. Fern and lessThan i greaterThan Chlorophytum tuberosum lessThan /i greaterThan (Roxb.) Baker. Crude extract of both these plant ( lessThan i greaterThan in vivo lessThan /i greaterThan as well as lessThan i greaterThan in vitro lessThan /i greaterThan ) were isolated with polar solvent n-hexane. Stigmasterol was quantified by HPTLC (toluene: methanol 9.5:0.5 [v/v] as mobile phase) following ICH (International Council for Harmonisation) guide lines at R lessThan sub greaterThan f lessThan /sub greaterThan (Retention factor) 0.34 which gave single peak at 540nm after derivatization. Method validation carried by applying samples on precoated silica gel 60 F lessThan sub greaterThan 254 lessThan /sub greaterThan TLC (Thin Layer Chromatography) plates and found out the precision, accuracy, specificity, reproducibility along with linearity. Standard stigmasterol was applied in the range of 4-9 µg/fractions. The intra-day precision value appeared to be 24.11% RSD (Relative standard deviation) concomitantly 24.77% RSD inter-day precision, that makes the method precise and reproducible. The limit of detection (LOD) - 4µg and Limit of Quantification - 12µg values for both the plant varieties observed to be accurate. The percentage recovery at values of three different levels was profoundly found to be more than 90%.This method clarified the knowledge of availability of stigmasterol in both plant varieties which would be beneficial for pharmaceutical industries. JITENDRIYA PANIGRAHI AND ILLA C PATEL Stigmasterol, Chlorophytum, HPTLC, Quantification 278-283