10.22376/ijpbs.2019.10.1.p1-12 Volume 8 Issue 1 2017 (January - March) Biochemical markers of bone turn over have been shown to provide valuable information for the diagnosis and monitoring of metabolic bone disease. These markers include bone resorption markers like urinary hydroxyproline, hydroxylysine, glycosides, pyridinoline, type I collagen telopeptides and bone formation markers like serum calcium, phosphorus, alkaline phosphatase and osteocalcin. Sixty postmenopausal women were taken as cases and forty premenopausal women were taken as controls. Serum calcium, serum alkaline phosphatase and urinary hydroxyproline were measured in both groups. Serum calcium was significantly decreased in postmenopausal women when compared to premenopausal women with a p value lessThan 0.01. Serum alkaline phosphatase and urinary hydroxyproline were increased in postmenopausal women compared to premenopausal women with a significant p value lessThan 0.01. This study was undertaken to diagnose at the earliest osteoporotic changes in postmenopausal women by the easily available, reliable and cost effective colorimetric methods. A.L.MANOHARI AND D.S.S.GIRIJAVANI Menopause, Hydroxyproline , Osteoporosis, Calcium 683-686