10.22376/ijpbs.2019.10.1.p1-12 Volume 8 Issue 2 2017 (April - June) The leaves of lessThan i greaterThan Piper betle lessThan /i greaterThan (Paan), have been shown to have anticancer and antimicrobial properties due to the presence of rich bioactive components, with the most predominant being eugenol. The present study deals with the synthesis of silver nanobioconjugates from the methanolic extract of lessThan i greaterThan Piper betle lessThan /i greaterThan leaves and its active compound eugenol. The presence of eugenol in the leaf extract was confirmed by HPLC analysis in the methanolic extract of betel leaves. Since various conditions influence the rapid reduction of Ag lessThan sup greaterThan + lessThan /sup greaterThan to Agº, different methods such as microwave heating, water bath heating at 60ºC, sunlight exposure and incubation at 37ºC were applied for the synthesis of nanobioconjugates. The antimicrobial efficiency of the synthesized silver nanobioconjugates from both leaf extract and active compound eugenol were also evaluated on Gram positive ( lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan ) and Gram negative ( lessThan i greaterThan Shigella flexneri lessThan /i greaterThan ) microorganisms. The results showed that the components in lessThan i greaterThan Piper betle lessThan /i greaterThan leaves and active compound eugenol act as good reductants in synthesizing silver nanobioconjugates and these nanobioconjugates exhibit good antimicrobial properties. R. PREETHI AND P. R. PADMA Piper betle leaves, eugenol, silver nanobioconjugates, antimicrobial activity 97-105