10.22376/ijpbs.2019.10.1.p1-12 Volume 8 Issue 2 2017 (April - June) Experiments were carried out to determine the effect of different plant growth regulating hormones on lessThan i greaterThan in vitro lessThan /i greaterThan propagation of lessThan i greaterThan Artemisia absinthium lessThan /i greaterThan L. from leaf explants. Organogenic callus was observed when explants were inoculated on the MS medium supplemented with BAP, 1.0 mg/l in combination with 2,4-D, 0.5mg/l. Maximum adventitious (28) shoots were induced on MS medium supplemented with NAA, 1.0 mg/l and KIN, 2.0 mg/l after the fifth subculture. MS medium augmented with 2 mg/l gave maximum root initiation, the average number of roots per shoot were 3.9. Individual plantlets were removed from the flasks, washed and transferred to a sterilized pot having soil:sand:decomposed coffee husk at a ratio (v/v) of 1:1:2 kept at high humidity (80-90%) for 15 days. RAPD-based SCAR marker was also developed lessThan i greaterThan lessThan /i greaterThan using 25 random primers for initial screening. A 475 bp polymorphic band obtained with an OPAA-8 primer which was specific to all lessThan i greaterThan A. absinthium lessThan /i greaterThan accessions. This RAPD-amplicon was eluted, sequenced and cloned and a pair of SCAR primers was designed. A single, bright, distinct band of 225 bp was obtained which shows its specificity towards the accessions of lessThan i greaterThan A. absinthium lessThan /i greaterThan and thus can be useful in its authentication. SHIPRA RANI JHA, MOHD MUGHEES, SHABNAM AKHTAR, JAVED AHMAD AND ALTAF AHMAD Artemisia absinthium, authentication, in vitro propagation, medicinal plant, SCAR marker 90-99