<b>Detection of synthetases genes involved in non ribosomallipopeptides (nrlps) biosynthesis from bacillus species by bioinformatics and pcr degenerated primers and estimation of their production. (Funded Research Article)</b>

10.22376/ijpbs.2019.10.1.p1-12

Volume 8 Issue 2 2017 (April - June) <b>Detection of synthetases genes involved in non ribosomallipopeptides (nrlps) biosynthesis from bacillus species by bioinformatics and pcr degenerated primers and estimation of their production. (Funded Research Article)</b> Non Ribosomal Lipopeptides NRLPs produced by lessThan i greaterThan Bacillus lessThan /i greaterThan spp. were firstly detected by bioinformatics in 54 sequenced genomes available from NCBI, which revealed the presence of the four lipopeptides families; surfactin, fengycin/plipastatin, iturin and kurstakin in 19 different strain, fusaricidin from ( lessThan i greaterThan Paenibacillus lessThan /i greaterThan ) lessThan i greaterThan B. lessThan /i greaterThan lessThan i greaterThan polymyxa lessThan /i greaterThan SC2 and unknown PKS-NRPS lipopeptide from lessThan i greaterThan B. lessThan /i greaterThan lessThan i greaterThan safensis lessThan /i greaterThan FO-36b . Also the absence of NRLPs synthetases genes were detected in 14 different strain, while the 21 other strains harbor different NRP synthetases genes. Secondly, we used the degenerated primers approach to detect the synthetases genes involved in lipopeptides biosynthesis in 9 lessThan i greaterThan Bacillus lessThan /i greaterThan strains. This technique detected non ribosomal synthetases genes for kusrstakin in lessThan i greaterThan B. thrunigenesis israelienne lessThan /i greaterThan NRRL HD-522, surfactin and plipastatin synthetases genes in lessThan i greaterThan B. subtilis lessThan /i greaterThan ATCC 21332 and unknown PKS-NRPS synthetases genes in ( lessThan i greaterThan Lysinibacillus lessThan /i greaterThan ) lessThan i greaterThan B. sphearicus lessThan /i greaterThan 23268 T. Thirdly, production levels were estimated for these 9 strains by HPLC. The production of lessThan i greaterThan B. amyloliquefaciens lessThan /i greaterThan FZB42 were 294, 62 and 210 mg.L lessThan sup greaterThan -1 lessThan /sup greaterThan surfactin, fengycin and mycosubtilin, respectivelly. lessThan i greaterThan B. amyloliquefaciens lessThan /i greaterThan S499 were 872, 103 and 103 mg.L lessThan sup greaterThan -1 lessThan /sup greaterThan surfactin, fengycin and bacillomycin respectivelly. lessThan i greaterThan B. subtilis lessThan /i greaterThan ATCC 21332 were 1060 and 226 mg.L lessThan sup greaterThan -1 lessThan /sup greaterThan of surfactin and plipastatin respectivelly. While, lessThan i greaterThan B. subtilis lessThan /i greaterThan 168 has no production. The production of lessThan i greaterThan B. licheniformis lessThan /i greaterThan ATCC 14580 strain was 358 mg.L lessThan sup greaterThan -1 lessThan /sup greaterThan of lichenysin, and lessThan i greaterThan B. pumilus lessThan /i greaterThan was produced 642 mg.L lessThan sup greaterThan -1 lessThan /sup greaterThan of pumilacidin. Also, the strains of lessThan i greaterThan B. thuringiensis kurstaki lessThan /i greaterThan and lessThan i greaterThan B. thuringiensis israelienne lessThan /i greaterThan NRRL HD-522 were produced 102 and 62 mg.g lessThan sup greaterThan -1 lessThan /sup greaterThan cells respectivelly of kurstakin. WALAA HUSSEIN AND SAMEH FAHIM Lipopeptides families, Bacillus spp., degenerated primers, NRLPs, lipopeptides production. 116-125