10.22376/ijpbs.2019.10.1.p1-12 Volume 8 Issue 2 2017 (April - June) Cancer is considered to be one of the leading causes of morbidity and mortality worldwide. For the past years, plants were known to possess metastatic tumor activities against numerous neoplastic cell lines. Also, nature merchandise represents a reservoir of various templates and it is being tapped to source novel metastatic tumour agents. lessThan i greaterThan Tabernaemontana divaricata lessThan /i greaterThan has been employed in Ayurvedic formulations in recent days. Leaves and flowers of lessThan i greaterThan T. divaricata lessThan /i greaterThan are employed in folk medicines by the Ayurvedic practitioners. It is a native of India and also different tropical regions. It may be a common plant that has been used historically for the treatment of various diseases. Also, the plant has been reported to be helpful for the treatment of cuts, inflammation, eye and skin diseases, wounds, diarrhea, ulcer and cancers. The aim of this study is to investigate the lessThan i greaterThan in vitro lessThan /i greaterThan metastatic antitumour activity of methanolic extract on lessThan i greaterThan T.divaricata lessThan /i greaterThan against HT-29 human carcinoma cell line. The methanolic extracts of the leaves and flowers of lessThan i greaterThan T.divaricata lessThan /i greaterThan were examined for its effect on HT-29 cell line. It was administered by exploitation of Tryphan blue exclusion technique. The toxicity of lessThan i greaterThan T.divaricata lessThan /i greaterThan against HT-29 cell was evaluated by MTT assay. The cell morphology of drug treated HT-29 cell line was studied by nuclear staining technique. From the performed assays, methanolic extract of those drugs show more anticancer activity on colon cell line (HT-29). S. SUBHASHINI AND M. PANDIMA DEVI Tabernaemontana divaricata, Tryphan blue exclusion technique, anticancer activity 316-323