10.22376/ijpbs.2019.10.1.p1-12 Volume 8 Issue 2 2017 (April - June) In this study we reported the screening of ten different fungal isolates for higher chitinase yield and its effect against the stored pest lessThan i greaterThan Corcyra cephalonica lessThan /i greaterThan as natural bio-control agent without toxic effects. Different fungal strains were cultured and screened the chitinase activity for the production of chitinase enzyme. The majority of the farmers profoundly rely on synthetic pesticides for the eradication of stored food pests. These synthetic pesticides are leaving a very large amount of toxic residue in the environment which is harmful to human beings. Due to these toxic effects of synthetic pesticide we attempted to find the biological control agent against stored pests. From the fungal populations, ten different fungal species were used to screen for the higher production of chitinase. The chitinase was partly purified by ammonium sulfate precipitation method and was evaluated for its anti-feedant and growth inhibitory activities against the stored food pest lessThan i greaterThan Corcyra cephalonica lessThan /i greaterThan Stainton at 62.5, 125, 250, 500 and 1000 ppm enzyme concentrations. lessThan i greaterThan Trichoderma viride lessThan /i greaterThan shows a maximum chitinase activity of 2.75±0.029 U.mL lessThan sup greaterThan -1 lessThan /sup greaterThan colloidal chitin on the sixth day. Next to lessThan i greaterThan Trichoderma viride, Metarhizium anisoplea lessThan /i greaterThan and lessThan i greaterThan Candida albicans lessThan /i greaterThan shows a chitinase activity of 2.51±0.031 and 2.23±0.070 U.mL lessThan sup greaterThan -1 lessThan /sup greaterThan Colloidal chitin respectively. Treatment of lessThan i greaterThan Corcyra cephalonica lessThan /i greaterThan with the partially purified chitinase from lessThan i greaterThan Trichoderma viride lessThan /i greaterThan reduced the normal larval development according to a dose-dependent manner. This study shows lessThan i greaterThan Trichoderma viride lessThan /i greaterThan chitinase can affect the growth of lessThan i greaterThan Corcyra cephalonica lessThan /i greaterThan larvae. The outcome necessitates further insect bioassays and detection of underlying mechanisms. NITHIN VIJAYAKUMAR, SANGILIMUTHU ALAGAR AND NALINI MADANAGOPAL Corcyra cephalonica, Trichoderma viride, DNS method, Biopesticide, Colloidal chitin 447-452