10.22376/ijpbs.2019.10.1.p1-12 Volume 8 Issue 2 2017 (April - June) Most of the essential plant nutrients stay as insoluble form in soil. The Phosphate Solubilizing Microorganisms are capable to solubilize inorganic phosphorus from insoluble compounds. A total of 24 phosphate solubilizing microbial colonies were isolated on Pikovskaya's agar medium containing insoluble tri-calcium phosphate (TCP) from rhizospheric soil. Out of 24 microbial isolates, 10 isolates showed highest Phosphate Solubilization Index (PSI) ranged from 2.25-3.80 were selected for further study. Among these 10 potent isolates, one strain (F2) showed highest phosphatase enzyme activity was 1.95µg/min/ml at day 5. The present investigation was carried out to study the effect of microbial biofertilizer on lessThan i greaterThan Vigna radiata lessThan /i greaterThan (mung) plant and the highest plant growth promotion was recorded when seeds were treated with bacteria, yeast and fungi (T3). S. S. KOLEKAR, P. D. DESAI, H. K. PANCHAL AND K. B. SHAH Phosphate solubilizing microorganisms, Phosphatase enzyme, Microbial biofertilizer and Vigna radiata plant 750-756