10.22376/ijpbs.2019.10.1.p1-12 Volume 8 Issue 2 2017 (April - June) As a part of our ongoing investigation on high altitude medicinal plants, we investigated lessThan i greaterThan Tanacetum gracile lessThan /i greaterThan (Asteraceae) by bioassay guided fractionation for antibacterial and antioxidant activity. The methanolic extract of the plant was fractionated into lessThan i greaterThan n lessThan /i greaterThan -hexane ( lessThan i greaterThan n lessThan /i greaterThan -Hex), dichloromethane (DCM), ethylacetate (EtOAc) and aqueous fractions. The antibacterial activity of the fractions was evaluated against three Gram negative viz., lessThan i greaterThan Escherichia coli lessThan /i greaterThan , lessThan i greaterThan Acinetobacter sp lessThan /i greaterThan . and lessThan i greaterThan Serratia sp lessThan /i greaterThan ., and two Gram positive including lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan and lessThan i greaterThan Enterococcus sp. lessThan /i greaterThan MDR clinical isolates. As, oxidative stress is a major cause of cognitive, cardiovascular diseases and cancer, and plant is the main source of antioxidant compounds, we evaluated the antioxidant potential of the fractions by chemical assays including total phenolic and flavonoid content, DPPH scavenging potential and FRAP assays. The results showed that EtOAc fraction possesses significant antibacterial activity against lessThan i greaterThan Serratia lessThan /i greaterThan sp. and also commendable antioxidant activity (DPPH IC lessThan sub greaterThan 50 lessThan /sub greaterThan 37.38 ± 0.4 μg/mL) comparable to that of vitamin C. Phytochemical screening of the fractions revealed the presence of phenolics including flavonoids in the fraction. The results may offer the use of the plant fraction as herbal remedy in the therapy of pathological conditions related to multi drug resistant microbes and generation of free radicals. Further phytochemical analysis in this regard could lead to some new drug candidates. MANISHA BHATNAGAR, ANUPAMA SHARMA AVASTHI AND SABARI GHOSAL Tanacetum gracile, Multi drug resistant (MDR), antibacterial activity, antioxidant activity 914-921