International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 8 Issue 3
2017 (July - September)
Dehairing of cattle hide by keratinase enzyme of Aspergillus flavus s125
The keratinase enzyme produced by the fungus lessThan i greaterThan Aspergillus flavus lessThan /i greaterThan S125 for depilation of skin was investigated. Dip method was adopted for enzymatic dehairing in which dried hide pieces were incubated with freshly extracted enzyme. Chemical processing was also performed simultaneously. During enzymatic dehairing the complete dehairing was observed after 12 hr at pH 9, while in chemical process, dehairing took 12 hr, at a high pH 11. Scanning electron micrographs of the dehaired skins showed the superiority in the quality of the enzymatically dehaired skin in comparison with the chemically treated one. Collagenolytic activity of the enzyme was also tested, as collagen is an important component of the hide and it was interesting to note that the keratinase produced by lessThan i greaterThan Aspergillus flavus lessThan /i greaterThan S125 was highly keratnolytic and while showing no collagenolytic activity. Dehairing at pH 9 was economical and environment friendly as it avoids the dumping of toxic effluents into the environment. The results of the present study revealed that keratinase isolated from lessThan i greaterThan Aspergillus flavus lessThan /i greaterThan S125 has the potential to replace chemical dehairing process, in addition to producing superior quality leather.
MINI.K.D, SAMPATHKUMAR S, AND JYOTHIS MATHEW
keratinase, Aspergillus flavus S125, depilation, dehairing and collagenolytic.
204-209