International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 8 Issue 3
2017 (July - September)
Extraction and optimization of Microalgal Carbonic Anhydrase for biosequestration of Carbon Dioxide
In the global effort to combat the predicted outcomes, several CO lessThan sub greaterThan 2 lessThan /sub greaterThan capture and storage technologies are being deliberated. One possibility rests on the use of carbonic anhydrase enzyme, which have been long known to accelerate the hydration of neutral aqueous CO lessThan sub greaterThan 2 lessThan /sub greaterThan molecules to ionic bicarbonate HCO lessThan sub greaterThan 3 lessThan /sub greaterThan lessThan i greaterThan − lessThan /i greaterThan species. The carbonic anhydrase (CA) activity were determined in two fresh water green algae lessThan i greaterThan Chlorella lessThan /i greaterThan sp lessThan i greaterThan . lessThan /i greaterThan and lessThan i greaterThan Scenedesmus pectinatus lessThan /i greaterThan growing in laboratory condition. In the detailed experiment, the effects of various parameters such as nitrate, pH, salinity, phosphate and light intensity were studied. The study reveals that the activity of enzyme is induced by alkaline pH (6.5-7.5), nitrate, salinity, phosphate concentration in the range of 250 mg/l, 25mg/l and 175 mg/l K lessThan sub greaterThan 2 lessThan /sub greaterThan HPO lessThan sub greaterThan 4 lessThan /sub greaterThan and 75mg/l KH lessThan sub greaterThan 2 lessThan /sub greaterThan PO lessThan sub greaterThan 4 lessThan /sub greaterThan respectively. Although the experiments were carried out under controlled experimental conditions, they could provide some preliminary data that would prove useful for the control of algal bloom in nature.
L. BENEDICT BRUNO, S. SANDHYA, J. DEEPIKA, RAVI SHANKAR
Chlorella sp., Carbonic anhydrase, CO2 capture, Microalgae, carbon concentration
954-965