10.22376/ijpbs.2019.10.1.p1-12 Volume 8 Issue 4 2017 (October - December) MicroRNAs (mi-RNAs) are conserved biomolecules that regulate gene expression at level of translation. miRNA play a critical role at various levels in an organism. This is to deduce mi-RNAs by computational methods where they are compared for homology in EST sequences of an organism. The lessThan i greaterThan Helianthus tuberosus lessThan /i greaterThan called as father of plant migration, having number of silent genes during its acclimatization to new world. A genomic approach is used to classify and target various identifiers at the nucleic acid level which govern the entire acclimatization of this plant. Raw Express Sequence Tags (ESTs) of lessThan i greaterThan Helianthus tuberosus lessThan /i greaterThan were downloaded from dbEST Database, after refinement, clustering, and assembly by TGICL program, contigs and singletons were generated which are stored in a local database. Mature miRNA and Stem Loop of lessThan i greaterThan Arabidopsis thaliana lessThan /i greaterThan were compared to generated local database by BLAST, were 50 mi-RNAs precursors were identified which aligned to multiple contigs where mature mi-RNA also showed their presence. The region of contigs alignment with the mi-RNA precursors were given in Mirfinder program for calculation of free energy values. Total 10 contig region identified where presence of mi-RNA precursor was feasible taking into consideration both the BLAST e-values and Mirfinder energy score. SUMIT GOVIL, SHAILESH KUMAR Contigs, Singletons, Clustering, Alignment, Assembly 166-172