10.22376/ijpbs.2019.10.1.p1-12 Volume 9 Issue 2 2018 (April-June) The present investigation is the comparative analysis of kaempferol from the field grown plant parts and lessThan i greaterThan in vitro lessThan /i greaterThan callus through which the enhancement of kaempferol by the influence of yeast elicitor were determined. The stem explants showed highest percentage of callus induction (74.34 + 0.67) and stem explants showed the highest callus growth in terms of fresh weight (3.90 + 0.05 g) and dry weight (0.41 + 0.01g). The maximum content of kaempferol in three type of samples was observed in this order; yeast elicitor treated samples greaterThan lessThan i greaterThan in vitro lessThan /i greaterThan raised callus samples greaterThan field grown samples. The highest amount of kaempferol was found in 2 ml yeast elicitor treated with stem callus sample (0.958 mg/g. dry weight). Hence, from the current investigations, it was proved that the highest amount of kaempferol was observed in elicitor treated lessThan i greaterThan in vitro lessThan /i greaterThan callus is provides resource for valuable drugs preparation against various diseases. DEVI MALIGA .K AND YOGANANTH .N Oxystelma esculentum, Asclepiadaceae, kaempferol, yeast elicitor, callus 135-139