10.22376/ijpbs.2019.10.1.p1-12 Volume 9 Issue 3 2018 (July-September) The genus lessThan i greaterThan Cleome lessThan /i greaterThan belongs to the family Cleomaceae. Most of the species of the genus are considered to be weeds but some are reported to possess nutritional and medicinal properties. The present investigation deals with the quantification of sinigrin, in the methanol extracts of two species of lessThan i greaterThan Cleome lessThan /i greaterThan , lessThan i greaterThan Cleome viscosa lessThan /i greaterThan L. and lessThan i greaterThan C. burmanni lessThan /i greaterThan W. & A. using HPLC. Sinigrin is reported to be the precursor of the anticancer compound, allylisothiocyanate and possesses antimicrobial property. HPLC analysis confirmed the presence of sinigrin in both the species. lessThan i greaterThan Cleome viscosa lessThan /i greaterThan contained higher amounts of sinigrin (21.35mg/g extract) than lessThan i greaterThan C. burmanni lessThan /i greaterThan (17.02mg/g extract). Eventhough, sinigrin has been subjected to extensive research in recent years, isolation protocols have not yet been standardised for sinigrin. The two species of lessThan i greaterThan Cleome lessThan /i greaterThan can serve as alternative sources for the isolation of sinigrin due to their abundance and availability. LAKSHMI S. PILLAI Cleome viscosa, Cleome burmanni, sinigrin, HPLC 47-51