10.22376/ijpbs.2019.10.1.p1-12 Volume 2 Issue 1 2011 (January - March) Bryonopsis laciniosa (L.) Naud. is a hjghly valuable medicinal plant belongs to the family Cucurbitaceae. It is used as an operient medicine and tonic. The objective of the present study was to achieve high frequency in – vitro rooting from explants like leaf,stem,nodal and cotyledon, explants derived calli and regenerated shoots on MS medium. Among the explants tested leaf and cotyledon cultures showed high frequency rooting on MS medium augmented with 2.0 mg/l BAP + 2.0 mg/l NAA and MS + 2.0 mg/l BAP + 2.0 mg/l IBA. The auxins2,4-D, NAA and IBA are used for rooting but NAA had found as the most effective hormone compared to IBA on root induction and elongation.  lessThan br / greaterThan V.J.E CAROLINE,B.MALLAIAH Bryonopsis laciniosa, Goniothalamin , High frequency rhizongenesis, NAAa – naphthalene acetic acid, IBA indole – 3 – butyric acid, BAP 6- benzylaminopurine. 216-223