International Journal of Pharma and Bio Sciences
ijpbs.net
editorijpbs@rediffmail.com (or) editorofijpbs@yahoo.com (or) prasmol@rediffmail.com
10.22376/ijpbs.2019.10.1.p1-12
Volume 9 Issue 4
2018 (October - December)
Purification and therapeutic potentialities of Phycocyanin in spirulina platensis
The aim of the present study is to purify and to find out the therapeutic potentialities of Phycocyanin(pc) present in lessThan i greaterThan Spirulinaplatensis. lessThan /i greaterThan Novel method for purifying the phycocyanin of lessThan i greaterThan S.platensis lessThan /i greaterThan was developed by freezing and thawing method. Antimicrobial assay was studied by agar well diffusion assay. The stability of the extracted pc was also confirmed by comparing its antioxidants using Ferric Reducing Antioxidant Potential (FRAP) and 2, 2'-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay. Effect of aqueous extract of lessThan i greaterThan S. platensis lessThan /i greaterThan was investigated on blood platelet aggregation in terms of prothrombin time (PT) and activated partial thromboplastin time (APTT).Two subunits of pc namely α-pc (15.6 kDa) and β-pc (23.4 kDa) were found to remain from the original mixtures based on SDS-PAGE. The results showed that all lessThan i greaterThan S. platensis lessThan /i greaterThan extracts exhibited great potential antibacterial activities against four bacterial and fungal strains. The study revealed that lessThan i greaterThan S. platensis lessThan /i greaterThan has potent bio compatibilities. The results of the study confirm that the pc can be used as drug, with lot of therapeutic ventures. All these studies confirm the therapeutic ventures of this compound as potent agent.
NEHA PURI, MANAS MATHUR, ANKITA ANAND, ASHISH SHARMA
AND AJIT KUMAR SWAMI
SDS-PAGE; Therapeutic ventures: Phycocyanin
57-68