10.22376/ijpbs.2019.10.1.p1-12 Volume 12 Issue 3 2021 (July-September) Bioactive substances are the leading-edge molecules in both the pharmaceutical and nutraceutical industries. Recently, bioactive compounds are gaining much importance for their enhanced resistance to numerous diseases and in boosting people's health by both traditional and modern ways of administration. In traditional medicine, many plants have provided valuable clues for being used as potential antiparasitic, antimalarial, leishmanicidal, anti-tumor, fungicidal, and antibacterial compounds. Still, several plants are yet to be screened for their abilities to stand forth as medicinal plants. Locally, Graviola (Gishta) fruits are being used for dietary purposes and traditional medicinal purposes, but their beneficial effects have never been attributed to the presence of active bio compounds. Hence, the present investigation was undertaken to study Gas Chromatography-Mass Spectrometry (GC-MS) analysis of methanolic extracts of Graviola seeds. GC-MS analytical method was employed with specific conditions such as Ion source temp 200°C, Interface temp 240°C, Scan range 40 – 1000 m/z, MS start time 5(min), MS end time 35 (min), Ionization EI (-70ev), Scan speed 2000. The methanolic extracts of Graviola seeds exhibited the presence of seventy types of bioactive compounds. The foremost important compounds identified are 1H-Cyclopenta [l, 3] cyclopropa [l, 2] benzene, octa hydro-7-methyl-3-methylene-4-( 1-methyl ethyl) -, [3aS-( 3a. alpha.,3b. beta., 4. beta., 7. alpha.,7 aS*)]-Bicyclo [4. 4. 0] dec-l-ene, 2-isopropyl-5-methyl-9-methylene-. gamma.-Muurolene. tetracosane, phytol and squalene. In an exceeding mass spectral view, each of the compounds has been identified based on their retention time and % of peak area(s). Analytes with high peak areas showed various pharmacological properties such as antibacterial, antifungal, anticancer, etc.,) which provided experimental evidence for its traditional medicinal claim. The spectra of phytochemical constituents found in the seed extracts signify the potential of the Graviola plant as a source of therapeutic agent(s) and may provide leads in the ongoing search for a novel phytotherapeutic agent. Harikrishna Ramaprasad Saripalli and Prasanna Kumar Dixit : Bioactive compounds, Methanolic extract, Gas Chromatography-Mass Spectrometry, Pharmacological activity, and Graviola seeds. 48-57