10.22376/ijpbs.2019.10.1.p1-12 Volume 14 Issue 3 July-September 2023 Nanomedicine is an emerging tool in biological science with a more prior application against no different disease conditions. The prefix 'nano' is a Greek prefix meaning something very small and near to around one thousand millionth of a meter (10−9 m). The American physicist and Nobel Prize laureate Richard Feynman introduced the concept of nanotechnology in 1959. During the annual meeting of the American Physical Society, Feynman presented a lecture entitled "There's Plenty of Room at the Bottom" at the California Institute of Technology (Caltech). This topic mainly emphasizes various Nano size ranges of medicine, especially Nanocarriers as Rigid & Non-rigid systems. Non-rigid nanocarrier systems are primarily of relatively soft structures that an external force can easily disrupt. Rigid nanoparticles are understood to retain the same form. Nanomedicines are employed in all different areas across the world in the field of Agriculture, Medicines & Cosmetics; Nanomedicines are best employed or designed for critical disease conditions such as Cancer, Diseases associated with the Brain, etc. Nanomedicine plays an active role in their therapeutic & diagnostic application. The selection of matrix materials depends on many factors such as Biocompatibility and toxicity, Degree of biodegradability, surface characteristics (charge and permeability), Antigenicity of the final product, Inherent properties of the drug (aqueous solubility and stability). The current topic particularly emphasizes different methods of preparation available in the market for Nanomedicine, Various methods of its characterization, such as Microscopy- Based Nanoparticle Characterization & Spectroscopy-Based Characterization Techniques. At the same time, High-resolution transmission electron microscopy imaging is an excellent methodology for differencing between micro or nano-crystalline diphasic and rigid polycrystalline and single crystalline phases. This methodology has been employed specifically for the surface study of nanostructure using the different chemical compositions of atoms and molecules in Nanoparticles to image the surface/body at the microscopic level. Ms. Pooja S Murkute, Dr. Mohammed Ismail Mouzam, Dr. Abubakar Salam Bawazir, Mr. Nakul P Kathar and Mr. Krishna K Deore Liposomes, Polymeric Nanoparticles, Solvent Diffusion method, High-resolution Transmission electron microscope, X-ray Photoelectron Spectroscopy, Dendrimers. 21-41