10.22376/ijpbs.2019.10.1.p1-12 Volume 2 Issue 3 2011 (July - September) Gemcitabine is an antineoplastic agent commonly used to treat some types of leukemia as well as cancers. In the present study, long circulating liposomal drug delivery system for Gemcitabine was developed, which will increase the efficacy and reduce the toxicity and target the organs (RES). Long circulating liposomal drug delivery system for Gemcitabine was prepared by thin film hydration method using two different carriers and same coating polymer with different concentrations. Four Formulations were prepared by using DPPC and DSPC as a carrier. All the formulations were compared in terms of particle size, polydispersity index, zeta potential, encapsulation efficiency, invitro release, in vivo tissue distribution and stability. Among those, Formulation F lessThan sub greaterThan 2 lessThan /sub greaterThan and F lessThan sub greaterThan 4 lessThan /sub greaterThan show better results. F lessThan sub greaterThan 2 lessThan /sub greaterThan and F4 were subjected to in vivo studies, where formulation F lessThan sub greaterThan 2 lessThan /sub greaterThan showed lower targeting efficiency towards liver spleen and heart, when compared to the formulation F4. Stability studies of F lessThan sub greaterThan 2 lessThan /sub greaterThan revealed that 4 lessThan sup greaterThan o lessThan /sup greaterThan C is the most suitable temperature for storage of long circulating liposomes. Arjun Goje, Rajendra C. Doijad And Sompur C.K Gemcitabine, leukemia, long circulating liposomal drug delivery, Dipalmityl phosphatidyl choline (DPPC), Distearoyl phosphatidyl choline (DSPC). 238-247