10.22376/ijpbs.2019.10.1.p1-12 Volume 2 Issue 3 2011 (July - September) Diabetes mellitus is a metabolic disorder and the management is an important criterion for pharmacotherapy. The medicinal plants play very important role in preventing the progress of the disease. Present study deals with screening of polyherbal extracts using lessThan i greaterThan in-vitro lessThan /i greaterThan techniques for its antidiabetic activity. The plants used in the study are lessThan i greaterThan Eugenia jambolana lessThan /i greaterThan and lessThan i greaterThan Cinnamomum zeylenicum lessThan /i greaterThan . The polyherbal extracts were subjected to lessThan i greaterThan in-vitro lessThan /i greaterThan evaluation using cell culture assays, α-glucosidase inhibition and antioxidant property. The cell lines 3T3L1 C2C12 and HepG2 were used to measure the uptake of glucose. The results showed that the glucose uptake was more in APKJ-004 polyherbal extract when compared with other extracts. α-glucosidase inhibitory activity was found in all the extracts and they were comparable to the standard acarbose and the highest inhibitory activity was found in the polyherbal extract APKJ004 (IC lessThan sub greaterThan 50 lessThan /sub greaterThan 147.2µg/ml). lessThan i greaterThan In-vitro lessThan /i greaterThan antioxidant potential revealed that the extract APKJ004 is more potent and is comparable to the standard. Based on lessThan i greaterThan in-vitro lessThan /i greaterThan evaluations, unique combination of glucose uptake activity and α glucosidase inhibition potential of extract APKJ-004 with prominent hypoglycemic activity will aid in the treatment of diabetes mellitus. A.Padmanabha Rao And Kaiser Jamil Anti diabetic Activity, cell lines, 3T3 L1, C2C12, HepG2, polyherbal extract, Glucose uptake, α-glucosidase 408-416