Isolation And Amplification Of Fem – A Gene From Mrsa Isolates

10.22376/ijpbs.2019.10.1.p1-12

Volume 2 Issue 3 2011 (July - September) Isolation And Amplification Of Fem – A Gene From Mrsa Isolates lessThan i greaterThan Statphylococcus aureus lessThan /i greaterThan is a Gram positive bacterium, which is naturally present in nasopharyngeal region of human beings and animals. After 1941, lessThan i greaterThan S.aureus lessThan /i greaterThan was developed drug resistant capacity to against all penicillin antibiotics. In 1961, Methicillin Resistant lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan were isolated. The present study Methicillin Resistant lessThan i greaterThan S. aureus lessThan /i greaterThan were isolated from clinical pus samples. All isolates were grown on Luria Bertani (LB) Agar containing Oxacillin antibiotic. Genomic DNA isolated from isolates using with standard procedure. lessThan i greaterThan Fem A lessThan /i greaterThan gene amplification using with Real Time PCR and suitable primers. The amplified gene was produced 510 bp at Agarose Gel Electrophoresis. S. Ganesh Manikandan, M. Hemalatha, C. Lakshminarasimhan And N. Thajuddin Methicillin Resistant Staphylococcus aureus, LB Agar,Real Time PCR, Fem A gene, Agarose Gel Electrophoresis. 28-35