10.22376/ijpbs.2019.10.1.p1-12 Volume 2 Issue 3 2011 (July - September) Allozyme analysis was used to assess genetic variability in tephritid fruit fly lessThan i greaterThan Bactrocera diversa lessThan /i greaterThan and lessThan i greaterThan B. zonata lessThan /i greaterThan . In our experimental conditions, allozyme analysis at twelve enzyme loci scores twenty one putative alleles. The amount of mean observed heterozygosity (H lessThan sub greaterThan O lessThan /sub greaterThan ) was 0.22 and 0.28 and amount of mean expected heterozygosity (H lessThan sub greaterThan E lessThan /sub greaterThan ) was 0.28 and 0.37, in lessThan i greaterThan B. diversa lessThan /i greaterThan and lessThan i greaterThan B. zonata, lessThan /i greaterThan respectively. The amounts of observed and expected heterozygosity were found to be higher as compared to the general low level of variability found in tephritid flies. Nei's genetic identity and distance value reveals very close similarity between the two species. Rashmi Singh, Akanksha Singh, Uma R. Agrawal And R.R.Tewari Allozyme, Bactrocera, Tephritidae, Heterozygosity, Molecular markers 193-199