10.22376/ijpbs.2019.10.1.p1-12 Volume 2 Issue 4 2011 (October - December) In vitro studies on the dorsal integumental melanophores of amphibian lessThan i greaterThan Hoplobatrachus tigerinus lessThan /i greaterThan revealed that the artificial UV-B radiation (313nm) at a dose range from 20.0 - 24.6 µW/cm lessThan sup greaterThan 2 lessThan /sup greaterThan for a continuous period of 60- 120 minutes caused an irreversible damage and disruption to the melanophores resulting into permanent clumping and aggregation of cells. However, at lower duration of exposure, the melanophores showed gradual pigment dispersion, most likely as an innate mechanism of defense to attenuate the UVB causing damage. Sharique A.Ali , Saima Salim, Ayesha S. Ali, And Jaya Peter Ultraviolet- B radiation; melanophores; amphibians; Hoplobatrachus tigerinus; UV defense. 158-173