10.22376/ijpbs.2019.10.1.p1-12 Volume 2 Issue 4 2011 (October - December) lessThan b greaterThan lessThan i greaterThan Objective lessThan /i greaterThan lessThan /b greaterThan : A pilot study to test the hypothesis that lessThan b greaterThan dysregulated expression of haemostasis genes in clonal granulocytes of MPD patients contributes to the genesis of haemostatic complications lessThan /b greaterThan . In this study of 36 patients with MPD and 18 healthy controls, specific target genes Procoagulant Tissue Factor [TF], prothrombotic cytokines Interlukin1 β [IL1β],Tumor Necrosis Factor α [TNFα],Vascular Endothelial Growth Factor[ VEGF] and Heparanase in clonal granulocytes were analyzed by qualitative methods, for disease associated aberrant gene expression, as proof of concept. lessThan b greaterThan lessThan i greaterThan Context lessThan /i greaterThan lessThan /b greaterThan : Granulocytes especially PMN leukocytes are well known mediators of thrombosis in inflammatory states and leukocytosis, a defining feature of MPD, correlates well with thrombosis, the risk falling with cytoreduction. Further, lessThan i greaterThan in vivo lessThan /i greaterThan activation of leukocytes and enhanced platelet-leukocyte interactions have been documented in MPD. Further research is needed to explore the links between leukocytes and the haemostatic complications of MPDs. lessThan b greaterThan lessThan i greaterThan Design lessThan /i greaterThan lessThan /b greaterThan : This observational study has compared patterns of gene expressions in granulocytes of patients with PV and ET by qualitative RT-PCR. Patients and Measurements: We studied 14 patients diagnosed with PV, 22 with ET at the Haematology department, and 18 healthy controls . Granulocytes were purified by Ficoll gradient centrifugation, RNA isolated and RT PCR performed using cDNA under optimized conditions. Finally PCR products were separated by Agarose gel electrophoresis and identified by UV transillumination. lessThan b greaterThan lessThan i greaterThan Results lessThan /i greaterThan lessThan /b greaterThan : TF was expressed by none of the controls but 42% of PV and 27% of ET patients granulocytes revealed TF expression. lessThan b greaterThan lessThan i greaterThan Conclusion lessThan /i greaterThan lessThan /b greaterThan : Upregulation of TF and other haemostasis related genes in granulocytes genes as suggested by this qualitative method of RTPCR suggests altered gene expression in granulocytes of MPD patients. This could be further explored by quantitative studies. Lakshmi Kannan, Mary Frances Mcmullin, Paul Winter RT PCR expression -TF,Heparanase,TNFα,IL-1β,VEGF and PAR-2 genes-granulocytes of PV and ET - hypercoagulabe state. 329-334